Tth111I PCR/RFLP marker in the canine rod transducin alpha (GNAT1) gene.

Source/description: PCR primers were designed based on the canine rod transducin alpha cDNA sequence (Genbank accession no. Z69597). A single nucleotide polymorphism (G/A) was found within the amplified sequence. A new primer was designed with a single nucleotide mismatch to create a Tth111I restriction site in the presence of the G allele (allele 2), but not the A allele (allele 1). The PCR product from genomic DNA is 397 bp. In the presence of the G allele the PCR product is cleaved into 379 and 18 bp restriction fragments.